Nanobodies, or VHH single-domain antibodies, are derived from heavy-chain only antibodies found in camelids. They gained increasing interest from researchers due to their potential use in research, diagnostic, and therapeutic applications. Advantages include their small size, high chemical and thermal stability, and ease of production.
Optimizing nanobody-target amine coupling based assays can be iterative and costly, especially with unstable ligands. Biacore™ cap-tag capture kit offers a reusable alternative. Attach your proteins to Series S Sensor Chip CAP via Biacore cap-tag.
We describe a streamlined workflow for characterization and rapid kinetic profiling of nanobody–target interactions using Biacore 1K+ SPR system, leveraging Biacore cap-tag capture kit and regeneration kit CAP. The predefined capture and regeneration conditions save an estimated half-day of instrument time and potentially several days of ligand attachment optimization. Initial kinetic estimates were obtained in under 5 hours, revealing a high-affinity interaction with slow dissociation kinetics. The Biacore cap-tag setup supported extensive chip reuse—up to 450 cycles—allowing for approximately 400 qualitative binding assessments or 200 full kinetic analyses on a single sensor chip. This method offers a cost-effective and scalable solution for early-stage screening and characterization in life sciences R&D.